Synergetic integration of laccase and versatile peroxidase with magnetic silica microspheres towards remediation of biorefinery wastewater

In this study, a tailor-made biocatalyst consisting of a co-immobilized lignolytic enzyme cascade on multi-functionalized magnetic silica microspheres (MSMS) was developed. Physical adsorption was the most promising strategy for the synthesis of individual immobilized laccase (IL), immobilized versatile peroxidase (IP), as well as co-immobilized laccase (Lac) and versatile peroxidase (VP) with an enzyme activity recovery of about 79, 93, 27, and 27.5%, respectively. Similarly, the biocatalytic load of 116, 183, 23.6, and 31 U/g was obtained for IL, IP, and co-immobilized Lac and VP, respectively. The co-immobilized enzyme system exhibited better pH stability than the free and individual immobilized system by retaining more than 100% residual activity at pH 7.0 after a 150-h incubation; whereas, the thermal stability and kinetics of the co-immobilized biocatalyst were not much improved. IL and IP could be recycled for 10 cycles after which they retained 31 and 44% of their initial activities. Co-immobilized Lac and VP were reused for ten consecutive cycles at the end of which Lac activity was depleted, and 37% of VP activity was left. Free enzymes, IL, IP, co-immobilized Lac, and VP were applied to biorefinery wastewater (BRW) in a batch study to investigate the transformation of phenolic contaminants over a period of 5 days. The major classes of phenolic constituents in terms of their order of removal in a Lac-VP system was phenol >2-chlorophenol > trichlorophenol > dichlorophenol > cresols > dimethylphenol >2 methyl- 4, 6-dinitrophenol > 4-nitrophenol > tetrachlorophenols > pentachlorophenol. The free enzymes and individually immobilized enzymes resulted in 80% dephenolization in 5 days. By contrast, the co-immobilized biocatalyst provided rapid dephenolization yielding the same 80% removal within 24 h and 96% removal of phenols in 60 h after which the system stabilized, which is the major advantage of the co-immobilized biocatalyst.

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Differentiating the effects of climate and land use change on European biodiversity: A scenario analysis

Current observed as well as projected changes in biodiversity are the result of multiple interacting factors, with land use and climate change often marked as most important drivers. We aimed to disentangle the separate impacts of these two for sets of vascular plant, bird, butterfly and dragonfly species listed as characteristic for European dry grasslands and wetlands, two habitats of high and threatened biodiversity. We combined articulations of the four frequently used SRES climate scenarios and associated land use change projections for 2030, and assessed their impact on population trends in species (i.e. whether they would probably be declining, stable or increasing). We used the BIOSCORE database tool, which allows assessment of the effects of a range of environmental pressures including climate change as well as land use change. We updated the species lists included in this tool for our two habitat types. We projected species change for two spatial scales: the EU27 covering most of Europe, and the more restricted biogeographic region of ‘Continental Europe’. Other environmental pressures modelled for the four scenarios than land use and climate change generally did not explain a significant part of the variance in species richness change. Changes in characteristic bird and dragonfly species were least pronounced. Land use change was the most important driver for vascular plants in both habitats and spatial scales, leading to a decline in 50–100% of the species included, whereas climate change was more important for wetland dragonflies and birds (40–50 %). Patterns of species decline were similar in continental Europe and the EU27 for wetlands but differed for dry grasslands, where a substantially lower proportion of butterflies and birds declined in continental Europe, and 50 % of bird species increased, probably linked to a projected increase in semi-natural vegetation. In line with the literature using climate envelope models, we found little divergence among the four scenarios. Our findings suggest targeted policies depending on habitat and species group. These are, for dry grasslands, to reduce land use change or its effects and to enhance connectivity, and for wetlands to mitigate climate change effects.     

Sediment processes and flow reversal in the undular tidal bore of the Garonne River (France)

A tidal bore is a series of waves propagating upstream as the tidal flow turns to rising, and the bore front corresponds to the leading edge of the tidal wave in a funnel shaped estuarine zone with macro-tidal conditions. Some field observations were conducted in the tidal bore of the Garonne River on 7 June 2012 in the Arcins channel, a few weeks after a major flood. The tidal bore was a flat undular bore with a Froude number close to unity: $\hbox {Fr}_{1} = 1.02$ and 1.19 (morning and afternoon respectively). A key feature of the study was the simultaneous recording of the water elevation, instantaneous velocity components and suspended sediment concentration (SSC) estimates, together with a detailed characterisation of the sediment bed materials. The sediment was some silty material ( $\hbox {d}_{50} \approx 13~\upmu \hbox {m}$ ) which exhibited some non-Newtonion thixotropic behaviour. The velocity and SSC estimate were recorded simultaneously at high frequency, enabling a quantitative estimate of the suspended sediment flux at the end of the ebb tide and during the early flood tide. The net sediment flux per unit area was directed upstream after the bore, and its magnitude was much larger than that at end of ebb tide. The field observations highlighted a number of unusual features on the morning of 7 June 2012. These included (a) a slight rise in water elevation starting about 70 s prior to the front, (b) a delayed flow reversal about 50 s after the bore front, (c) some large fluctuations in suspended sediment concentration (SSC) about 100 s after the bore front and (d) a transient water elevation lowering about 10 min after the bore front passage. The measurements of water temperature and salinity showed nearly identical results before and after the tidal bore, with no evidence of saline and thermal front during the study.     

Using small triangular baffles to facilitate upstream fish passage in standard box culverts

Environmental Fluid Mechanics - A culvert is a covered channel to pass streams and floodwaters through an embankment. The ecological impact of culverts has been recognised, in particular in terms...